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Please note that all translations are automatically generated. Click here for the English version.
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02:41 min
December 29th, 2023
DOI :
10.3791/201000-v
Transcript
通过为每个样品创建圆柱形锡箔来准备嵌入模具。用最佳切割温度或 OCT 化合物填充模具。然后准备两个保温桶。
在小桶中加入异戊烷,在大桶中加入液氮。在肌肉解剖前 10 分钟将小桶放入大桶内。首先准备小鼠进行解剖。
切开老鼠脸上的皮肤,露出咬肌。切除腮腺,使咬肌的后部完全暴露。将咬肌从其前附着物解剖到下颌骨上的后附着物。
然后将肌肉组织浸没在 OCT 中,使其垂直于化合物。使用针架将模具转移到预冷的异戊烷中。等待 40 秒,直到 OCT 变为白色和纯色。</
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