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Please note that all translations are automatically generated. Click here for the English version.
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04:10 min
June 23rd, 2023
DOI :
10.3791/201002-v
Transcript
首先,向HeLa细胞中加入0.25%胰蛋白酶-EDTA溶液,并在37摄氏度下孵育两到三分钟。通过移液轻轻解离细胞,并以 1 比 4 的比例将它们接种到新平板中以进行细胞传代。为了将质粒转染到 HeLa 细胞中,将 1 μg 经验证的 PX458 sgRNA 质粒与 50 μL 还原血清培养基混合在试管 A.In 管 B 中,轻轻混合 2 μL 转染试剂和 50 μL 还原血清培养基,并在室温下孵育 5 分钟。
然后,将试管 A 和 B 中的内容物混合,并在室温下再孵育五分钟。将
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