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Please note that all translations are automatically generated. Click here for the English version.
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04:02 min
November 17th, 2023
DOI :
10.3791/201012-v
Transcript
首先,将根癌农杆菌细胞培养物条纹转化为选择性抗生素补充的酵母甘露醇琼脂平板。将板在28摄氏度的立式培养箱中孵育过夜。第二天,将培养物中的菌落接种在12.5毫升酵母甘露醇Luria-Bertani肉汤混合物上,并在28摄氏度的轨道振荡器上以250RPM孵育16小时。
用抗生素接种 12.5 毫升农杆菌菌株LBA4404 112.5 毫升酵母甘露醇肉汤中。在28摄氏度的轨道振荡器上以250RPM孵育培养物16小时。用培养液将过夜培养物调节至光密度 0.4。
然后在孵育前
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