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Please note that all translations are automatically generated. Click here for the English version.
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02:57 min
December 29th, 2023
DOI :
10.3791/201021-v
Transcript
首先,在 37 摄氏度下解冻分离的血浆样本。将每个血浆样品的 100 微升转移到 1.5 毫升管中。向每个试管中加入一毫升无钙HBSA缓冲液。
将样品以20,000g离心15分钟,温度为4摄氏度。将上清液吸至20微升,而不干扰沉淀。接下来,向每个试管中加入一毫升无钙HBSA缓冲液。
然后上下移液以重新配制沉淀。涡旋每个管几秒钟,以确保细胞外囊泡的均匀分布。然后在4摄氏度下以20,000g离心15分钟。
再次,在不干扰沉淀的情况下将上清液吸入 20 微升。
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