Extracellular Vesicle Tissue Factor Assay with Blood Plasma Derived Extracellular Vesicles

To begin, defrost the isolated blood plasma samples at 37 degrees Celsius. Transfer 100 microliters of each plasma sample into 1.5-milliliter tubes. Add one milliliter of calcium-free HBSA buffer to each tube.

Centrifuge the samples at 20, 0