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Please note that all translations are automatically generated. Click here for the English version.
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02:37 min
December 15th, 2023
DOI :
10.3791/201034-v
Transcript
首先,在室温下解冻实时荧光定量PCR试剂盒中提供的所有试剂。解冻后,以中等速度脉冲涡旋组分 10 秒以混合它们。然后,在室温下以8, 000G离心10秒。
将解冻的组件放在冷却块上。接下来,为HBV阳性样品、五种HBV标准品和无模板对照准备PCR混合物。轻轻移液 7 至 10 次,然后脉冲涡旋,在室温下以 8, 000G 离心 10 秒,彻底混合添加的试剂。
然后,将15微升PCR混合物移液到每个PCR管中。加入 15 微升提取的 DNA 样品、五种 HBV 标准品
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