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Please note that all translations are automatically generated. Click here for the English version.
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04:23 min
July 28th, 2023
DOI :
10.3791/201036-v
Transcript
首先打开图像J2.1.0中的延时线粒体图像。通过导航到菜单栏并单击图像,根据视野分离位置堆栈。然后,转到“复制”,输入“切片”或“帧号”,然后单击“确定”。接下来,使用自由选择工具在单元格周围绘制感兴趣的区域。
要运行宏,请打开图像 J,导航到菜单栏,然后单击插件、宏和运行。然后,选择清除背景并保存图像的宏。要确定像素大小和体素深度,请打开图像 J 并选择图像。
访问菜单栏,选择“图像”,然后单击“属性”。确定像素大小和体素深度后,使用自由选择工具绘制包含整个单元格
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