To begin, warm up a fluorescent spectrophotometer for 15 to 30 minutes before measurement. Then click on measure, and set the integration time to 0.1 seconds, increments to one nanometer, slit width to one nanometers, and the signal formula to S1-CR1-C. Next, use a Pasteur pipette to carefully transfer 3.5 milliliters of a diluted Curcuma longa extract into a quartz cuvette.
Measure the emission spectra with a 365 nanometer excitation source, and set the emission range from 380 nanometers to 625 nanometers. Measure the excitation spectrum of the sample with the wavelength of highest emission. Set the lower limit for the excitation range at 330 nanometers, and the upper limit set to the monitored emission wavelength minus 15 nanometers.
Remeasure the emission spectrum of the sample with the highest excitation wavelength. Set the emission range starting at the excitation wavelength plus 15 nanometers up to 625 nanometers. Next, set the excitation range fixed between 330 and 435 nanometers, and the emission between 450 and 650 nanometers for all dilutions of Curcuma longa extract.
Then clean the cuvette with ethanol, and measure the emissions of the remaining dilutions. To measure the emission excitation matrix of Chitosan, set the slit width to one nanometer and the integration time to 0.1 seconds, the emission ranges from 300 to 370 nanometers, and the excitation range from 385 to 450 nanometers. Then transfer the Chitosan solution to a washed cuvette, and place it in the spectrophotometer to measure its emission excitation matrix.
Place a multi-tester fabric over the ATR crystal of the FTIR instrument. Then measure the IR transmittance of the fabric. To perform fluorescence analysis of the Chitosan-dyed fabric, place the fabric in the sample holder of the instrument.
Fix the fabric position in the middle of the window with glass slides. Now set the integration time to 0.1 seconds, increments to one nanometer, slit width to 0.6 nanometers, and signal formula to S1C-R1C. Then set the emission range between 380 to 635 nanometers, and measure the fluorescence at 365 nanometers.
Use the wavelength of highest excitation as determined by the photoluminescence analysis to measure the emission spectrum of the sample. Add 15 nanometers to the excitation wavelength, and set it as the lower limit for the emission range. Set the upper limit to 625 nanometers.
Lastly, measure the emission spectra of one to 50 diluted Chitosan-finished Curcuma longa dyed fabrics at 365 nanometers. To perform morphological analysis of fabrics, first mount a handheld UV source of 365 nanometers on an iron stand. Point it towards a stereo microscope.
Next, place the fabric on the stage, and open the white light source. Set the zoom to the lowest magnification to locate the target imaging area. Increase the magnification to four times, and refine the image focus with the fine adjustment knob.
With the built-in imaging software, insert a scale bar and capture the image. To ensure uniform imaging, set the exposure compensation to 100, the exposure time to 100 milliseconds, and the gain to 20. Then adjust the hue values of red to 27, green to 32, and blue to 23.
Lastly, adjust the sharpness to 75, de-noise to 35, saturation to 50, gamma to six, and contrast to 50. Switch on the ultraviolet lamp after turning off the white light source. Now capture the image with the same imaging parameters for all fabrics.
UV analysis of the multi-test fabrics showed the successful deposition of the Curcumanoid solution at different concentrations. The photoluminescent emission spectra of Curcumanoid Chitosan-dyed fabrics showed enhanced optical properties.
