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Please note that all translations are automatically generated. Click here for the English version.
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01:36 min
December 22nd, 2023
DOI :
10.3791/201053-v
Transcript
首先,将麻醉的小鼠放在手术台上。使用无菌眼科剪刀和镊子,打开胸腔和腹腔。这将暴露心脏和肠道。
仔细地分别解剖肠系膜动脉和主动脉。在PBS中冲洗肠系膜动脉,并将它们转移到含有1%青霉素 - 链霉素 - 两性霉素B溶液的10厘米培养皿中。同样,冲洗主动脉并将其转移到另一个含有相同溶液的培养皿中。
快速去除主动脉和肠系膜动脉周围的所有脂肪。冲洗两次后,将动脉置于组织培养罩中的立体显微镜下。小心地沿着动脉做一个纵向切口。
然后用细镊子撕下主动脉的外层和肠系膜动脉
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