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01:59 min
December 22nd, 2023
DOI :
10.3791/201055-v
Transcript
为了表征从小鼠主动脉和肠系膜动脉中分离的CD34 +干细胞,进行组织块培养并通过磁分离获得细胞。为了检测分化后成纤维细胞标志物表达中的内皮细胞,用 488 纳米的激光激发在 40 倍放大镜下观察细胞。胰蛋白酶消化细胞后,通过离心将其沉淀并重悬于 100 微升分选缓冲液中。
然后将它们与抗小鼠 CD16/CD32 单克隆抗体在 4 摄氏度下孵育 5 分钟。然后加入 2 μL 每种所需单克隆抗体进行免疫荧光染色,并在 4 摄氏度下再次孵育 10 分钟。用一毫升缓冲液洗涤细胞两次。<
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