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Please note that all translations are automatically generated. Click here for the English version.
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01:32 min
January 12th, 2024
DOI :
10.3791/201059-v
Transcript
首先,在两毫升微量离心管中加入所需量的拟南芥哥伦比亚零种子。对于种子的表面灭菌,在试管中加入70%乙醇两分钟,然后轻轻摇晃试管。除去乙醇后,用无菌水清洗种子五分钟。
从试管中取出水,让种子在重力作用下沉淀到试管底部,然后再去除剩余的水。要创建种子水悬浮液,请在装有种子的试管中加入等体积的无菌水。使用宽移液器吸头,按照播种网格在培养基板上的每个区域连续均匀分布约 30 至 40 粒种子。
让水在种子中干燥 30 分钟。用微孔胶带密封板,并用铝箔包裹。在黑暗中将种子在4
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