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Please note that all translations are automatically generated. Click here for the English version.
24 Views
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03:18 min
December 1st, 2023
DOI :
10.3791/201206-v
Transcript
首先将冻干的 MOG 35 55 肽重新悬浮于水中。在实验前,在包衣缓冲液中将肽储备液稀释至每毫升 10 微克。现在将 100 微升的最终溶液移液到 96 孔板的孔中。
同时用溶解在包被缓冲液中的 100 微升 BSA 包被相同数量的孔。用胶膜密封板以防止蒸发,并将板在 4 摄氏度下孵育过夜。第二天,用 200 微升 PBS 补充 0.5% tween 20 对板进行三次洗涤。
随后,每孔加入 100 微升由 3% BSA 组成的 PBS 溶液。将密封板在 37 摄
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