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Please note that all translations are automatically generated. Click here for the English version.
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01:26 min
December 15th, 2023
DOI :
10.3791/201212-v
Transcript
首先,在行为测试后获得安乐死远端 MCAO 小鼠模型的解剖大脑,并将其放入零下 20 摄氏度的冰箱中 20 分钟。然后将大脑定位到 1 毫米的大脑矩阵中。并使用 microtone 刀片将大脑切成两毫米厚的冠状动脉切片。
将脑切片转移到 24 孔培养板中,并添加 2% TTC 以覆盖脑组织。将板在 37 摄氏度下孵育 30 分钟。然后小心地将脑切片在 4% Paraldehyde 溶液中固定 15 分钟。
使用扫描仪,对大脑切片进行光学扫描。脑组织学分析显示远端 M
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