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Please note that all translations are automatically generated. Click here for the English version.
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03:14 min
January 19th, 2024
DOI :
10.3791/201233-v
Transcript
首先,设置落射荧光显微镜。成像前,将 Stage Top 培养箱预热至 37 摄氏度,并以每分钟 0.02 升的速度在腔室中运行加湿的二氧化碳。然后将含有受感染单层的载玻片放入培养箱室并密封盖子。
在明场照明下使用 20X 物镜,选择单层内视场的 X 和 Y 坐标。优化成像参数并使用 488 纳米的激发波长获取图像。为最大限度地减少光毒性,请将光源设置为 50% 功率,曝光时间为 50 毫秒。
确保没有像素饱和,并进行调整以检测荧光钙传感器的整个动态范围。设置成像环
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