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Please note that all translations are automatically generated. Click here for the English version.
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02:01 min
May 3rd, 2024
DOI :
10.3791/201247-v
Transcript
一旦美国农业部检查的肉店的去核猪眼到达实验室,就进行外部组织去除。用一只手握住附着在眼睛上的肌肉。使用手术刀,用另一只手轻轻切掉巩膜周围的组织。
使用弯曲的虹膜剪刀修剪视神经。然后将眼睛放在冰上,角膜朝下。对于解剖,使用镊子将眼睛转移到含有10%聚维酮碘溶液的孔中并旋转以确保其完全被覆盖。
将无菌纱布放在培养皿的浅倒盖上。将细胞过滤器放入单独的培养皿中。将眼睛从聚维酮碘溶液转移到含有DPBS的孔中,并清洗约五秒钟。
然后逐渐将其移至下一个含DPBS的孔中
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