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Please note that all translations are automatically generated. Click here for the English version.
39 Views
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03:35 min
May 3rd, 2024
DOI :
10.3791/201248-v
Transcript
首先,对从美国农业部检查的肉店获得的去核眼睛进行消毒和解剖,以准备眼罩。轻轻地将眼罩(内部朝上)放入装有细胞过滤器的培养皿中。将冷却的 DPBS 添加到眼罩中,确保液位刚好低于切口的最低点。
在手电筒下,找到神经视网膜开始与视网膜色素上皮分离的任何区域,然后用钝镊子轻轻抓住抬起的神经视网膜并将其剥离。轻轻收集视盘附近的神经视网膜。使用连接到真空抽吸系统的牧草移液器,吸出神经视网膜。
小心地添加额外的冷却 DPBS 以更换吸气体积。现在,吸出这个添加的 DPBS 以去
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