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02:57 min
October 6th, 2023
DOI :
10.3791/201249-v
Transcript
首先,取用荧光团偶联的 EV-microRNA 和细胞 microRNA 转染的 Calu6 细胞。通过圆底聚苯乙烯FACS管随附的35微米过滤器盖过滤细胞悬浮液。然后设置流式细胞仪,打开流式细胞仪,让它预热30分钟后再使用。
用鞘液灌注流体系统。之后,验证并调整激光对准。为了进行质量控制,将超纯过滤水装入流式细胞仪并以适当的流速运行,以确保足够的采集时间。
然后设置从 FSC 5, 000 处捕获 Calu6 细胞信号的阈值。为了解释荧光团之间的光谱重叠,使用未转
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