Flow Cytometry Analysis of Cell-miRNA and EV-miRNA Signal in Calu6 Cells Transfected with Fluorophore-Conjugated EV-miRNA(Video)

To begin, take Calu6 cells transfected with fluorophore-conjugated EV-microRNA and cell-microRNA. Filter the cell suspension through a 35 micrometer strainer cap included with the round bottom polystyrene FACS tubes. Then set up the flow cytometry