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Flow Cytometry Analysis of Cell-miRNA and EV-miRNA Signal in EVs Isolated from Calu6 Cells Transfected with Fluorophore-Conjugated EV-miRNA

80 Views

•

03:01 min

•

October 6th, 2023

DOI :

10.3791/201250-v

October 6th, 2023

•
80 Views
1Department of Biological Sciences, Purdue University, 2Purdue Institute for Cancer Research, Purdue University

Transcript

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Flow Cytometry
Extracellular Vesicles
EV miRNA
Cell miRNA
Fluorophore conjugated MiRNA
Calu6 Cells
Nano flow Cytometry
Performance Beads
Instrument Calibration
Gating
Sample Preparation
Data Analysis

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A Flow Cytometry Protocol for Understanding Export Mechanisms
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