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Please note that all translations are automatically generated. Click here for the English version.
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03:19 min
January 12th, 2024
DOI :
10.3791/201253-v
Transcript
要开始 G/I 拖尾,在冰上制备含有总 RNA、尾部缓冲液混合物和尾部酶混合物的 20 微升混合物。在 37 摄氏度下在热循环仪中孵育 60 分钟。然后,加入止尾溶液,在冰上保持两分钟。
进行逆转录和PCR扩增。对PCR产物进行高分辨率凝胶电泳后,使用软件访问数据。打开 XAD 文件,然后在“树视图”面板中选择样品名称或梯形图。
放大和缩小电泳图和凝胶状图像,以进行详细显示。要获得峰尺寸,请打开所选样品的电泳图。右键单击电泳图,然后选择手动积分,通过拖动水平线手动选
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