Flow Cytometry Analysis of Transfected Mouse Pluripotent Stem Cells (mPSCs)

After transfection of mPSCs, aspirate the media from the six-well plate. Then add 200 microliters of trypsin into each well. Swirl and incubate for 30 seconds at 37 degrees Celsius.

Tap the side of the plate and add 200 microliters of ice co