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Click Assay for the Measurement of Histone Acetyltransferase 1 Acetylation

58 Views

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04:18 min

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January 26th, 2024

DOI :

10.3791/201342-v

January 26th, 2024

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58 Views

Shreenidhi Rajkumar¹, Danielle Dixon¹, Andrew M. Lipchik², Joshua J. Gruber¹

¹Departments of Internal Medicine and the Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, ²Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University

Transcript

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Histone Acetyltransferase 1 Acetylation

Biotinylated Histone H4 Peptide

Acetylation Reactions

4 Penten Oil Co enzyme A

BSA Pre blocked Black NeutrAvidin coated 96 well Plates

THPTA Copper Mixture

Sodium Ascorbate

Streptavidin Horseradish Peroxidase

Amplex Red Detection Reagents

Hydrogen Peroxide

Sodium Phosphate

From the series

Development of a Click-Based Assay for Screening HAT1 Enzyme Activity

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