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Please note that all translations are automatically generated. Click here for the English version.
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04:18 min
January 26th, 2024
DOI :
10.3791/201342-v
Transcript
首先,重悬合成的肽,该肽在DMSO中浓度为0.1毫克/毫升,作为阳性对照。将重悬肽与生物素化组蛋白 H4 肽混合以创建标准曲线。接下来,将乙酰化反应一式两份组装在96孔PCR板中。
20 微升反应应包括 10 微升酶、1 微升 H4 肽、1 微升 20X 缓冲液和 1 微升 DTT。向每个孔中加入一微升DMSO或测试化合物。轻轻移液混合混合物。
然后将混合物在冰上孵育 10 分钟,以允许酶抑制剂络合。为了继续乙酰化反应,将两微升 4-戊烯油辅酶 A 与四微升水混合。
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