Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
TR - Turkish
JA - Japanese
Please note that all translations are automatically generated. Click here for the English version.
61 Views
•
02:29 min
December 1st, 2023
DOI :
10.3791/201345-v
Transcript
从麻醉的受体小鼠中提取天然肝脏后,使用笔镊轻轻抓住受体的肝门静脉或PV边缘,并用虎钳固定。将一根八哦的丝线绕在 PV 周围,并在其上放置一个血管夹。在距 PV 边缘约 0.5 毫米处创建四分之一的圆周截面。
将生理盐水通过孔时,使用专用仪器扩大孔。用直镊子夹住袖带手柄并将其插入管腔。然后用 8.0 丝线固定袖口。
从 PV 中取出血管夹,以允许肝脏再灌注。最后,小心地松开笔镊,完成PV重建。定位肝叶后,将附着在受体IHIVC背侧的笔镊移动到移植物的右下叶。
Sign in or start your free trial to access this content
Explore More Videos
From the series
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Librarians
Access
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved