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Please note that all translations are automatically generated. Click here for the English version.
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01:02 min
January 26th, 2024
DOI :
10.3791/201346-v
* These authors contributed equally
Transcript
首先,获得原发性肺肿瘤或肺癌脑转移组织,将肿瘤组织包埋在成对的石蜡块中,并使用微量将其切成四微米厚的部分。将切片放入水中。使用镊子选择最好的一种,并将其粘附在聚赖氨酸涂层载玻片上。
为了增强组织粘附,将载玻片在 65 摄氏度的烤箱中孵育 30 分钟。脱水时,将载玻片浸入二甲苯中,然后浸入浓度降低的乙醇溶液中,然后用离子水洗涤三分钟。接下来,将载玻片浸没在柠檬酸钠缓冲溶液中。
在高压锅中将载玻片置于高温高压下,并在蒸馏水中冷却至室温三分钟。最后,用PBS清洗载玻片三次
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