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Please note that all translations are automatically generated. Click here for the English version.
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03:05 min
January 26th, 2024
DOI :
10.3791/201367-v
Transcript
首先,从小鼠身上分离出股骨、胫骨、骨盆和肱骨。用手术刀切开长骨的生长板,切除骨骺。将其放入 10 厘米的培养皿中,加入 M199 培养基和 2%FBS 在冰上。
接下来,使用 28 号针头和 10 毫升注射器填充 M199 培养基和 2% FBS,将骨髓冲洗到冰上的 15 毫升管中。将冲洗的骨头与骨骺一起放入培养皿中。将 15 毫升管直立在冰上,直到骨髓沉淀。
然后取出 M199 培养基加 2% FBS,将 4 毫升 B&M 解离混合物加入 15 毫升管中,并在 3
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