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03:36 min
January 12th, 2024
DOI :
10.3791/201389-v
Transcript
首先,将传导导管仪器的压力体积传感器浸入0.9%氯化钠溶液中。传感器饱和后,连接实验装置。按软件上的启动图标可自动记录来自压力体积传感器的监测数据。
接下来,使用 Mikro-Tip 压力容积软件校准压力和电导率。将麻醉的大鼠固定在等温加热板上,保持背部与板接触。用凡士林涂覆温度探头,然后将探头插入大鼠的直肠。
现在,在大鼠脖子上正中线的右侧做一个四厘米长的切口。使用镊子分离肌肉和结缔组织。将颈动脉与其他组织隔离,然后在颈动脉下方放置三条 5/0 手术线。
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