mass spectrometry following the 2d-lc operation on aconitum pendulum samples

使用分子联网的 2D-HPLC-MS 分析藏药植物 APB

Tibetan medicine is an integral part of traditional Chinese medicine, adhering to the principles of Tibetan medical practices, and is used for disease prevention and treatment1. However, Tibetan herbal medicine contains complex plant chemical constituents characterized by significant fluctuations in content. Limited understanding of the fundamental bioactive elements has become a bottleneck in the modernization of Tibetan medicine2. The application of liquid chromatography-mass spectrometry (LC-MS), combining the strong separation power of chromatography with the high sensitivity of mass spectrometry (MS), has been widely used in natural medicine analysis3. However, due to the limitation of a single separation mechanism, components with highly similar structures tend to coelute in one-dimensional liquid chromatography separation. In subsequent mass spectrometric analysis, the low-abundance co-eluted components are difficult to detect due to ion suppression caused by high-abundance components4.
2D-HPLC, which stands for two-dimensional high-performance liquid chromatography, represents a novel chromatographic method that harmoniously combines different separation mechanisms using a pair of columns. This includes the fusion or alternation of normal-phase chromatography with reversed-phase liquid chromatography and hydrophilic interaction liquid chromatography with reversed-phase liquid chromatography5. By merging these complementary chromatographic properties, the enhanced separation capability is achieved, effectively addressing the challenges caused by complex sample matrices6. Furthermore, by coupling two-dimensional chromatography with MS, the powerful separation ability of 2D-HPLC and the high sensitivity detection capability of MS can be fully integrated, providing support for the study of complex drug systems and their fundamental constituents7,8,9.
Tibetan medicine commonly features a multifaceted array of components and functionalities, where active ingredients typically exist in intricate compositions and at minimal concentrations. By integrating 2D-LC as a robust separation system and MS as an exceedingly sensitive detector, the challenges posed by intricate samples in terms of separation and identification can be more effectively addressed10,11,12. This amalgamation substantially contributes to advancing the exploration of the chemical composition of Tibetan medicine.
Regardless of whether it is traditional LC-MS or 2D-LC-MS, a vast amount of information can be obtained. However, extracting structural information of complex system components from this massive amount of information has always been a significant challenge. Therefore, researchers have developed various methods for screening and mining MS data. Global Natural Products Social Molecular Networking (GNPS) is an MS/MS data organization and visualization platform where 2D-LC-MS mass spectrometry data can be uploaded13. Each spectrum is considered as a vector and compared to all other spectra using cosine similarity. When the similarity between two spectra exceeds a threshold, they are connected in a molecular network (MN). This can be used for the rapid identification of known compounds and the determination of various unknown natural products14.
Tibetan medicine usually has multiple functions, but its complex composition and significant concentration differences make it difficult to effectively elucidate the relationship between function and material basis15. An in-depth study of Tibetan medicine requires the systematic characterization of as many components as possible. In the framework of this study, we intend to use APB in Tibetan medicine as the research object to demonstrate the strategy and process of systematically studying the complex chemical components of Tibetan medicine using 2D-HPLC-MS technology and MN technology. In the construction of the 2D chromatographic system, we combined reversed-phase liquid chromatography and hydrophilic interaction liquid chromatography with significant separation mechanisms to achieve more effective separation of the complex components of APB16. In addition, to overcome solvent incompatibility between the two dimensions, an at-column dilution modulation mode was adopted. By combining the powerful separation capability of 2D-LC with the high sensitivity detection capability of MS, the spectral information of the complex components in APB is obtained more effectively and comprehensively. Furthermore, through the network and visualization of massive spectral information by MN technology, the components of APB are systematically analyzed. The strategy and process demonstrated in this study are expected to be applied to the study of other Tibetan medicines, promoting research on the material basis of Tibetan medicine, which is of great significance for advancing Tibetan medicine resources and improving quality control standards for Tibetan herbs17. The overall experimental process is shown in Figure 1.
In the experiment presented here, a new at-column dilution modulator was introduced into the Agilent two-dimensional liquid chromatography (2D-LC) system18. By adding an independent delivery pump, the flow path of the analysis was changed, resulting in a high orthogonality of the 2D-LC analysis. The coupling and switching between the two dimensions are accomplished by two six-port valves, as shown in Figure 2. When one sample loop is filled in the first dimension, another sample loop is analyzed in the second dimension. This means that the filling time of the 1D loop and the running time of the 2D are equal. This requires the fast gradient generated by the Binary pump in the second dimension. No peaks are lost when the entire effluent is analyzed. This is particularly helpful for the analysis of unknown samples. It results in a large number of 2D chromatograms that need to be combined for data analysis.

作者聚焦：在自然医学分析中集成 2D-HPLC-MS 和分子网络

2D-HPLC-MS技术结合分子网络鉴定藏药乌头成分

In this study, we employed a comprehensive approach utilizing 2D-HPLC-MS technology in conjunction with molecular networking to unravel the intricate chemical composition of the Tibetan medicinal plant APB. We want to establish a more efficient method for identifying the chemical composition of natural medicines. The main challenging of the current experiments is that the mass spectrometry section is not equipped with high-resolution mass spectrometry, resulting in the detection of different components.2D-LC-MS enables more accurate identification and the quantification of the compounds. Complex mixtures based on sample separation, combined with molecular network technology, it can be better process complex data information and it improves the efficiency and the accuracy of any size. Our laboratory will focus applying existing technologies to get the separation of natural, medicinal, chemical components.

对 乌头摆 样进行 2D-LC 操作后的质谱分析

首先，打开真空泵的开关。打开氩气瓶主阀和分压阀，将压力调节至0.3兆帕左右。要设置 MS 方法，请输入运行时间（以分钟为单位）到 93.00，并输入值以配置采集时间、极性、质量范围、传输值数量、传输值持续时间等。根据屏幕上显示的值设置扫描事件详细信息。要设置数据相关设置，请使用禁用的单独极性设置。将中性损失设置在前三名至三名，将产品批量列表设置在前三名内。保存以将设置配置为仪器方法。单击序列设置按钮以打开序列表。在表单中输入样品类型、文件名、路径、样品ID、仪器方法、位置、进样量等信息。保存信息以记录序列表。然后单击“开始分析”按钮以设置并开始 MS 采集。

mass-spectrometry-following-2d-lc-operation-on-aconitum-pendulum

Research

JoVE Journal

Medicine

Mass Spectrometry Following the 2D-LC Operation on Aconitum pendulum Samples

2D-HPLC-MS Technology Combined with Molecular Network for the Identification of Components in Tibetan Medicine Aconitum pendulum

In this study, a comprehensive approach was employed, utilizing 2D-HPLC-MS technology in conjunction with the molecular network to unravel the intricate ...

科研

医学

Sample Preparation for 2D-HPLC-MS of Aconitum pendulum

乌头摆2D-HPLC-MS的样品制备

Implementing 2D-Liquid Chromatography on Aconitum pendulum Samples for Enhanced Compound Separation

在 乌头摆 样上实施 2D 液相色谱以增强化合物分离

Integration of Molecular Network Operation to 2D-HPLC-MS for the Analysis of Aconitum pendulum Samples

将分子网络操作集成到 2D-HPLC-MS 中，用于乌 头 摆样的分析

对 乌头摆 样进行 2D-LC 操作后的质谱分析

对乌头摆样进行 2D-LC 操作后的质谱分析