Preparation of Electrocompetent Cells to Achieve Single-Copy Gene Expression in an Efflux-Deficient Bacterial Strain

To begin place all sterile, 1.5 milliliter microfuge tubes and sterile electroporation cuvettes on ice. Then place the bottle of sterile water, that was stored at four degrees Celsius, on ice. Transfer 1.5 milliliters of the overnight bacterial cu