Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
TR - Turkish
JA - Japanese
Please note that all translations are automatically generated. Click here for the English version.
9 Views
•
01:42 min
December 29th, 2023
DOI :
10.3791/201443-v
Transcript
首先,获得宿主酵母菌株,用诱饵质粒转化。将突变体文库引入其中后,选择具有 500 至 2000 个菌落的板。将两张滤纸放在复制块上,制作板的几个复制品,并在 30 摄氏度下将板孵育一到两天。
对于酵母两种杂交颜色测定,将一张滤纸放在复制板上,直到其完全湿润。用几张纸巾去除多余的水分,两次。然后,使用镊子从边缘撕下滤纸从板上剥下,并迅速将其浸入液氮中。
在干燥的纸巾上解冻冷冻的滤纸,菌落面朝上。解冻后,立即将其转移到另一张浸有缓冲液的滤纸上。盖上装有滤纸的塑料托盘,将
Sign in or start your free trial to access this content
From the series
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Librarians
Access
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved