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01:48 min
March 1st, 2024
DOI :
10.3791/201460-v
Transcript
为了制备拟南芥Thaliana幼苗的培养基,将一半强度的Murashige和Skoog或MS培养基与2%蔗糖和0.9%琼脂混合。灭菌后,将培养基倒入方形培养皿中,确保避免风干以保持湿度。将拟南芥种子浸入含有一毫升无菌水的两毫升微量离心管中,温度为四摄氏度,超过12小时。
然后用一毫升 2% 次氯酸钠溶液对种子进行灭菌两分钟。用无菌水彻底清洗种子六次,以去除次氯酸钠溶液。使用无菌的一毫升尖端,将种子播种在MS琼脂培养皿上。
用透气的医用胶带密封培养皿,并将其垂直放置在
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