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Please note that all translations are automatically generated. Click here for the English version.
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01:05 min
March 1st, 2024
DOI :
10.3791/201461-v
Transcript
将 Bacillus velezensis 细胞接种到无菌的 Luria-Bertani 培养基中,并在 37 摄氏度下以 170 RPM 振荡孵育,直到 600 纳米处的光密度达到 0.8 至 1.2。将细胞悬浮液以 6, 000 G 离心两分钟,然后将沉淀重新悬浮在 PBS 缓冲液中。最后一次洗涤后,将颗粒重新悬浮于新鲜的半强度MS培养基中,使其最终光密度为600纳米。
将细菌培养物稀释 100 倍,直到光密度达到 0.01。将稀释的细菌悬浮液接种到含有拟南芥幼苗的六孔板中
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