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Please note that all translations are automatically generated. Click here for the English version.
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01:42 min
March 8th, 2024
DOI :
10.3791/201482-v
Transcript
首先,获得与适当的质粒一起施用的安乐死小鼠。对于培养皿制备,将 1.9 毫升培养基加入玻璃培养皿中,并小心地将细胞培养插入物放在其上。然后将 500 微升培养基添加到过滤器中并将其置于冰上。
取出胚胎并将它们放入含有冰冷PBS的培养皿中。在显微镜下,根据绿色荧光蛋白或其他荧光标记物的表达选择胚胎。解剖大脑后,将其放入冷冻模具中取出的熔融琼脂糖凝胶中,并滚动数次。
琼脂糖在室温下凝固后,将其置于冰上。使用振动切片机,将大脑冠状切成350微米的厚度,然后将它们排列在过滤
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