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Please note that all translations are automatically generated. Click here for the English version.
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02:10 min
February 16th, 2024
DOI :
10.3791/201488-v
Transcript
首先,将人肠道样体 (HIE) 传代所需的所有材料安排到工作平台上。将HIEs与生长因子还原的3D培养基质混合。将混合物接种到 24 孔板上,每孔 3 滴 10 微升 3D 培养基质,并在 37 摄氏度下孵育 20 分钟。
然后加入 350 微升 HIE 生长培养基。七天后,从四个孔中收集HIE到1.5毫升管中。将 HIE 以 2000g 离心 40 秒以除去碎屑。
使用移液器,从试管中取出过多的3D培养基质。将 0.5 毫升冷 PBS 加入管中。使用 1 毫升胰岛
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