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Please note that all translations are automatically generated. Click here for the English version.
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01:13 min
April 12th, 2024
DOI :
10.3791/201492-v
Transcript
首先,在适当的培养基中培养斑马鱼。然后每天收集样本,以鉴定每个阶段的无菌鱼。然后在 TSA 和血液 TSA 板上涂覆 20 至 50 微升的废培养基样品。
在有氧条件下在 30 摄氏度下孵育板。同样,在厌氧条件下将废物培养基包被在琼脂平板上,并将它们置于厌氧 30 摄氏度培养箱中。对于液体培养,将 200 微升样品加入含有 BHI 或 TSB 的需氧管和厌氧管中,并将它们放入振荡培养箱中。
最后,每隔几个时间观察板和肉汤的无菌性并记录结果。无菌测试显示,从无菌斑马鱼
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