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Please note that all translations are automatically generated. Click here for the English version.
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04:29 min
March 1st, 2024
DOI :
10.3791/201502-v
Transcript
首先,将 500x 橙色染料添加到 PBS 溶液中以获得不同的染料比例,在桌面上启动数据采集软件。单击“仪器”,然后选择相应的微孔板型号,然后选择“确定”。单击“采集”设置,然后选择“荧光”。
在波长接口下,将 lambda one 设置为 470 纳米和 570 纳米,然后按 Okay。将每种浓度的橙色染料吸取 100 微升到 96 孔板的孔中。将板放入荧光酶标仪的检测台。
单击软件上的“读取”图标,在室温下以两分钟的间隔测量每种染料浓度的荧光 13 次。每次确定
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