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プロテインGゲルによるトランスフェクションHEK293タンパク質のプレクリアとエピトープタグアフィニティーゲルを用いたタンパク質複合体の捕捉(英語)

30 Views

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01:17 min

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January 5th, 2024

DOI :

10.3791/201504-v

January 5th, 2024

•
30 Views
1Department of Molecular and Internal Medicine, Graduate School of Biomedical & Health Sciences, Hiroshima University

Transcript

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Preclearing
Transfected HEK293 Protein
Protein G Gel
Capturing Protein Complexes
Epitope Tag Affinity Gel
Protein Sample
Protein G Gel Slurry
Rotator
Centrifuge
Supernatant
Sample Buffer
Epitope Tag Slurry

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共免疫沈降法を用いたHEK-293細胞のタンパク質相互作用解析
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