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Protein G Gel을 사용한 transfection된 HEK293 단백질의 사전 클리어링 및 Epitope tag Affinity Gel을 사용하여 단백질 복합체 포획

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01:17 min

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January 5th, 2024

DOI :

10.3791/201504-v

January 5th, 2024

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30 Views

Hiroko Shinjo¹, Gaku Nagano¹, Shogo Ishii¹, Natsumi Himeno¹, Yutaro Yamamoto¹, Junji Sagawa¹, Ryuta Baba¹, Gentaro Egusa¹, Noboru Hattori¹, Haruya Ohno¹

¹Department of Molecular and Internal Medicine, Graduate School of Biomedical & Health Sciences, Hiroshima University

Transcript

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Transfected HEK293 Protein

Capturing Protein Complexes

Epitope Tag Affinity Gel

Protein G Gel Slurry

Epitope Tag Slurry

From the series

Co-immunoprecipitation을 사용한 HEK-293 세포의 단백질 상호 작용 분석

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