synthesis of lignin submicron particles for bioflavonoid delivery

Síntesis de partículas micro/submicrónicas de lignina para la liberación de bioflavonoides

Nowadays inclination towards biopolymers such as cellulose, chitosan, collagen, dextran, gelatin, and lignin as precursors for the design of micro-/submicron carriers with customizable size, physicochemical properties, and biofunctionalities has increased in the biomedical, pharmaceutical, and food technology industries due to their applicability in tissue engineering, 3D bioprinting, in vitro disease modeling platforms, packaging industry, emulsion preparation, and nutrient delivery among others1,2,3.
Novel studies highlight the aspects of lignin-based hydrogels as well as micro- and nano- formulations4 as advantageous vehicles used for food packaging materials5, energy storage6, cosmetics7, thermal/light stabilizers, reinforced materials, and drug-carrier matrices8 for the delivery of hydrophobic molecules, improvement of UV barriers9, as reinforcing agents in nanocomposites, and as an alternative to inorganic nanoparticles due to some recent safety issues10,11,12. The reason behind this tendency is the biocompatibility, biodegradability, and non-toxicity of the natural hetero biopolymer, as well as its proven bioactivities of lignin-antioxidant potential and radical scavenging, anti-proliferative, and antimicrobial activities13,14,15,16,17.
Scientific literature reports various methods for synthesis (self-assembly, anti-solvent precipitation, acid precipitation, and solvent shifting)18 and characterization of lignin-based micro-/nano- scaled formulations, including the application of expensive or harmful solvents such as tetrahydrofuran (THF), dimethyl sulfoxide (DMSO), N,N-dimethylformamide (DMF), and acetone, and complicated, indirect, and tedious processes that use a lot of equipment and toxic substances12,19,20.
To overcome the latter disadvantages, the following protocols present novel methodologies for the synthesis of lignin-based micro-/submicron particles using cheap and green solvents; easy, straightforward, quick, and sensitive processes requiring little equipment, non-toxic substances, and simple methods for their characterization and the determination of encapsulation capacity towards poorly water-soluble bioactive compounds and in vitro release potential of the lignin matrices. The presented lab-scale production methods are advantageous for the manufacture of functional lignin carriers with tunable sizes, high encapsulation capacity, and sustainable in vitro release behavior utilizing simple characterization procedures and eco-friendly chemicals that can find application in various areas of biomedical sciences and food technology. Two flavonoids were applied as target molecules encapsulated into the lignin particles: morin-into the microparticles, and quercetin-into the submicron particles. The difference in the structures of both flavonoids Is only the position of the second -OH group in the B-aromatic ring: the -OH group is on the 2&#39; position in morin and on the 3&#39; position in quercetin, thus both organic compounds are positional isomers. The latter fact presumes similar behavior of both bioactive natural compounds in the processes of encapsulation and/or release.

Autor destacado: Desarrollo y caracterización de micropartículas ecológicas basadas en lignina para mejorar la administración de bioflavonoides

Síntesis, caracterización, encapsulación y medición del potencial de liberación de nuevas partículas micro/submicrónicas de lignina alcalina

The objective of this research was to create a straightforward and environmentally friendly process to produce small lignin particles. The study aimed to analyze their physical, chemical, and structural properties, as well as investigate their ability to encapsulate and release bioflavonoids in stimulated gastrointestinal environments. Our method synthesizes lignin based particles using cheap and green solvents.The process is easy, quick, and requires minimal equipment and non-toxic substances. It also has simple methods for characterization, encapsulation, capacity determination, and in-vitro release potential of poorly water soluble bioactive compounds. The innovative biopolymer microparticles are suitable for oral administration due to the lower risk of gastric irritation.The submicron particles, due to the small size and significant release potential, could be applied as injectable formulations.

Síntesis de partículas submicrónicas de lignina para la administración de bioflavonoides

Para empezar, añade 125 miligramos de lignina alcalina en 25 mililitros de agua ultrapura y disuélvela con un agitador magnético. Agregue gradualmente un mililitro de etanol al 96% en la solución de lignina alcalina. Revuelva la mezcla en un agitador magnético a 500 RPM durante tres minutos.A continuación, disuelva 0,5 gramos de ácido cítrico en agua ultrapura hasta un volumen final de 50 mililitros para preparar una solución de ácido cítrico al 1%. Con una jeringa, agregue siete mililitros de ácido cítrico al 1% a la mezcla. Continúe revolviendo la mezcla durante 10 minutos hasta que una solución de color marrón claro se transforme en una suspensión turbia de color marrón claro.Ahora, utilizando un homogeneizador de ultrasonidos, homogeneice las micropartículas durante dos ciclos, cada uno de cuatro minutos a una intensidad del 96%, luego liofilinice las micropartículas a una temperatura de 64 grados centígrados en un liofilizador y guárdelas en un secador para su uso futuro. Para preparar micro o submicrónicas de lignina encapsuladas en flavonoides naturales, pese 0,04 gramos de quercetina, disuélvala en un mililitro de etanol y agregue esta solución etanólica a la solución acuosa de lignina alcalina.

synthesis-of-lignin-submicron-particles-for-bioflavonoid-delivery

Research

JoVE Journal

Chemistry

Synthesis of Lignin Submicron Particles for Bioflavonoid Delivery

74 vistas.  Trakia University. Para empezar, añade 125 miligramos de lignina alcalina en 25 mililitros de agua ultrapura y disuélvela con un agitador magnético. Agregue gradualmente un mililitro de etanol al 96% en la solución de lignina alcalina. Revuelva la mezcla en un agitador magnético a 500 RPM durante tres minutos.A continuación, disuelva 0,5 gramos de ácido cítrico en agua ultrapura hasta un volumen final de 50 mililitros para preparar una solución de ácido cítrico al 1%. Con una jeringa, agregue ...

Video: Síntesis de partículas submicrónicas de lignina para la administración de bioflavonoides

Green Synthesis, Characterization, Encapsulation, and Measurement of the Release Potential of Novel Alkali Lignin Micro-/Submicron Particles

The applicability of biopolymer micro-/nano- technology in human, veterinary medicine, pharmaceutical, and food technology is rapidly growing due to the great potential of biopolymer-based particles as effective carrier systems. The use of lignin as a basic heteropolymer biomatrix for the design of innovative micro-/submicron formulations allows the achievement of increased biocompatibility and offers various active functional groups presenting opportunities for customization of the physicochemical properties and bioactivities of the formulations for diverse applications. The aim of the present study was to develop a simple and ecofriendly methodology for the synthesis of lignin particles with micro- and submicron size; to evaluate their physicochemical, spectral, and structural characteristics; and to examine their capacity for encapsulation of biologically active molecules and potential for in vitro release of bioflavonoids in simulated gastrointestinal media. The presented methodologies apply cheap and green solvents; easy, straightforward, quick, and sensitive processes requiring little equipment, non-toxic substances, and simple methods for their characterization, the determination of encapsulation capacity towards the poorly water-soluble bioactive compounds morin and quercetin, and the in vitro release potential of the lignin matrices.

We describe novel, simple methodologies of synthesis and characterization of biocompatible lignin micro- and submicron particles. These formulations provide a facile approach for the utilization of the heteropolymer, as well as an alternative for the rational design of multifunctional carrier matrices with potential applicability in biomedicine, pharmaceutical technology, and the food industry.

The applicability of biopolymer micro-/nano- technology in human, veterinary medicine, pharmaceutical, and food technology is rapidly growing due to the ...