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Please note that all translations are automatically generated. Click here for the English version.
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03:36 min
April 26th, 2024
DOI :
10.3791/201531-v
Transcript
首先,将整个PEG沉淀的腺相关病毒HEK293培养物转移到一个大的锥形管中。在4摄氏度下以2,820g离心培养物15分钟。弃去上清液,并将沉淀重悬于15毫升含有钙和镁的PBS中。
将重悬部分转移到 50 毫升管中。加入 10 毫升 PBS 以收集剩余的 PEG 沉淀物,并将其全部转移到 50 毫升管中。然后加入 40 微升 DNase I,并在 37 摄氏度下孵育一小时。
使用玻璃巴斯德移液管,用 15.5 毫升浓缩细胞裂解物填充 25 毫米 x 89 毫米的多异构
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