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Iodixanol-Gradientenreinigung von transfizierten HEK293-Zelllysaten für die konzentrierte Adeno-assoziierte Virusproduktion

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03:36 min

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April 26th, 2024

DOI :

10.3791/201531-v

April 26th, 2024

•
78 Views
1Laboratory for Regeneration of Sensorimotor Systems, Netherlands Institute for Neuroscience, Royal Netherlands Academy of Arts and Sciences (KNAW), 2Centre for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije Universiteit Amsterdam

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Iodixanol Gradient
HEK293 Cell Lysates
Adeno associated Virus Production
PEG Precipitation
DNase I Treatment
Ultracentrifugation
Virus Extraction
Centrifugal Filtration
PBS
Sucrose

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Vereinfachte und effiziente AAV-Produktion mit HEK293-Suspensionskultur
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