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Purificação por gradiente de iodixanol de lisados celulares HEK293 transfectados para produção concentrada de vírus adenoassociados

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03:36 min

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April 26th, 2024

DOI :

10.3791/201531-v

April 26th, 2024

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78 Views

Kimberly L. Pietersz¹, Paul J.H. Nijhuis¹, Matthijs H.M. Klunder¹, Joëlle van den Herik¹, Barbara Hobo¹, Fred de Winter¹, Joost Verhaagen^1,2

¹Laboratory for Regeneration of Sensorimotor Systems, Netherlands Institute for Neuroscience, Royal Netherlands Academy of Arts and Sciences (KNAW), ²Centre for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije Universiteit Amsterdam

Transcript

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Iodixanol Gradient

HEK293 Cell Lysates

Adeno associated Virus Production

PEG Precipitation

DNase I Treatment

Ultracentrifugation

Virus Extraction

Centrifugal Filtration

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Produção simplificada e eficiente de AAV usando a cultura de suspensão HEK293

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