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Please note that all translations are automatically generated. Click here for the English version.
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03:51 min
April 12th, 2024
DOI :
10.3791/201547-v
Transcript
首先,将切开的 200 微升移液器吸头底部插入预制的微流体捕获装置中。移液器使用配备 0.2 微米过滤器的注射器将 400 微升 β-酪蛋白溶液注入芯片的入口储液器中。将芯片以 900 G 离心 6 分钟。
现在,入口和出口的液位应该相等。在两台物镜载玻片上画出垫片的轮廓。用高氧等离子体清洁载玻片一分钟,使其具有亲水性。
在载玻片顶部加入低胶凝温度的琼脂糖,直到它完全被琼脂糖覆盖。然后将载玻片倾斜 90 度角,并将多余的琼脂糖排到组织上。将载玻片置于 50 摄氏度下
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