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Please note that all translations are automatically generated. Click here for the English version.
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01:40 min
March 1st, 2024
DOI :
10.3791/201562-v
Transcript
首先将 10 至 15 个新出现的果蝇放入新鲜的标准培养瓶中。在 12 小时的光暗循环中将这些小瓶保持在 25 摄氏度和 60% 相对湿度。要准备用于保持刺激的玻璃毛细管,请选择硼硅酸盐玻璃毛细管,并使用移液器拉动器械拉动玻璃毛细管。
然后,将钨棒用于参比电极和记录电极。将这些电极反复浸入 10% 硝酸钾溶液或氢氧化钾溶液中几秒钟,使其锐化至一微米的尖端直径。要将一只苍蝇从小瓶中抽出到吸液器中,请小心地抽出吸液器,并将手指放在吸液器的末端以捕获苍蝇。
现在,将吸液器
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