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Please note that all translations are automatically generated. Click here for the English version.
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01:14 min
March 8th, 2024
DOI :
10.3791/201569-v
Transcript
首先,收集用过的聚碳酸酯管并彻底丢弃先前的样品。为防止样品干燥到管的一侧,立即将超速离心管浸入自来水中,然后在 0.1% 十二烷基硫酸钠或 SDS 中孵育管 10 分钟。从试管中倒出大部分(但不是全部)SDS溶液。
并使用棉签彻底擦拭具有细胞外囊泡颗粒的管子区域。冲洗时,用热自来水填充管子并完全倒出。三次冲洗后,使用喷雾瓶将软化水喷入管中,然后加入 70% 乙醇。
将管子倒置完全干燥,然后将其转移到干净的罐子中重复使用。
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