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Please note that all translations are automatically generated. Click here for the English version.
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04:50 min
May 31st, 2024
DOI :
10.3791/201603-v
Transcript
在将灭菌管连接到生物芯片之前,首先用 200 微升 PBS 冲洗管路,然后用 200 微升 EC 培养基冲洗管路。用新鲜制备的细胞培养基冲洗上腔室和下腔室后,将储液器插入生物芯片的出口侧,并将储液器与管子连接到生物芯片的入口。对于循环培养基的填充,将管道连接到储液器和生物芯片。
现在用 500 微升 EC 培养基填充储液罐。将生物芯片与管道连接,以每分钟 21 微升的流速开始灌注。第二天,停止大量涌入并清空水库。
在无菌安全柜下,将 500 μL EC 培养基移液到
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