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02:35 min
November 10th, 2023
DOI :
10.3791/201612-v
Transcript
兔眼经结膜剜除后,立即将50毫升4%多聚甲醛和PBS置于冰上。15 分钟后,将眼睛转移到 100 毫米解剖培养皿中,并用 PBS 填充培养皿以防止眼睛干燥。在解剖显微镜下,使用手术刀片,开始在角膜缘前方一毫米处切开一个切口,使其与虹膜平面平行。
将弯曲的剪刀插入初始切口,然后继续沿圆周切割,与角膜缘保持一毫米的距离。然后用镊子取出角膜,用实验室湿巾轻轻擦去PBS。将角膜在室温下置于 30% 蔗糖溶液中进行冷冻保护。
用弯曲的剪刀穿过虹膜进行初步剪裁。然后在眼睛周围沿
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