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Please note that all translations are automatically generated. Click here for the English version.
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02:29 min
November 10th, 2023
DOI :
10.3791/201613-v
Transcript
兔眼冷冻保护完成后,将其从30%蔗糖溶液转移到100毫米解剖培养皿中。在解剖显微镜下,小心地翻转眼睛,以去除眼睛内部多余的蔗糖溶液。使用湿巾轻轻擦去眼睛外部多余的蔗糖溶液,用最佳切割温度或 OCT 化合物填充一次性包埋模具至 0.5 至 1 厘米的深度。
将眼睛朝上放入嵌入模具中,然后慢慢用 OCT 化合物填充眼睛内部,避免气泡形成。一旦眼睛在OCT化合物中出现,并且不接触模具的内表面,就用石蜡包裹包埋模具,并在4摄氏度下储存过夜。第二天,将包埋模具放入解剖皿中。
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