Sucrose Density Gradient Based Purification of Lentivirus Particles for Gene Delivery

To begin, remove the plunger from a 50-milliliter syringe, and insert a low protein binding polyethersulfone or a polyvinylidene fluoride 0.45-micrometer sterile filter into the syringe end. Using a 25-milliliter serological Stripette, add the len