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Please note that all translations are automatically generated. Click here for the English version.
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01:20 min
March 15th, 2024
DOI :
10.3791/201642-v
Transcript
首先,从液氮中取出装有细胞的冷冻瓶,并在 37 摄氏度的水浴中解冻两分钟。将解冻的细胞转移到 15 毫升管中后,逐渐加入 10 毫升预热培养基并离心管。弃去上清液,将细胞重悬于一毫升补充的含有FBS和氯化钙的DPBS中。
接下来,将 1 毫升细胞悬浮液与 50 微升死细胞去除混合物和生物素选择混合物混合在 5 毫升聚苯乙烯圆底管中并孵育。涡旋后,向细胞悬液中加入 100 微升葡聚糖珠,并用移液管轻轻混合两次。然后向混合物中加入 1.3 毫升补充的 DPBS,并在室温下用磁铁孵育
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